| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myoblast determination protein 1;Class C basic helix-loop-helix protein 1;bHLHc1;Myogenic factor 3;Myf-3;MYOD1;BHLHC1, MYF3, MYOD; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MyoD1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AABF-13 is a rabbit monoclonal antibody that recognizes myoblast determination protein 1 (gene MYOD1) in human samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 34.5 kDa, and the supplier reports an observed band at about 50 kDa.
MyoD (MYOD1) is a bHLH transcription factor and master regulator of skeletal muscle identity. Together with Myf5 and myogenin it activates muscle-specific genes and can convert fibroblasts into myoblasts. It is a standard marker of activated satellite cells and committed myoblasts in myogenesis and muscle regeneration studies.
| Target | Myoblast determination protein 1 (gene MYOD1; UniProt P15172, human) |
|---|---|
| Clone | AABF-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.5 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human myoD1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places myoblast determination protein 1 in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
