| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myoglobin;MB; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Myoglobin, different from the related mouse sequence by three amino acids, and from the related rat sequence by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This antibody is intended for detection of MB (Acid-sensing ion channel 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Myoglobin/MB Antibody Picoband® catalog # A04058. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human Myoglobin, different from the related mouse sequence by three amino acids, and from the related rat sequence by six amino acids.
- Molecular weight context: reported MW: 17 kDa; calculated MW: 17184 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Acid-sensing ion channel 2; Myoglobin. Myoglobin (MB) also known as PVALB, is a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. It is a member of the globin superfamily and is expressed in skeletal and cardiac muscles. This gene is mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue (rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure. Functional note: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Reported localization: Cell membrane. Multi-pass membrane protein. Expression/tissue context: Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary.
Research relevance and current trends
- Cancer: Researchers commonly examine how MB (Acid-sensing ion channel 2) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how MB (Acid-sensing ion channel 2) relates to this theme using model systems and orthogonal readouts.
- Energy Metabolism: Researchers commonly examine how MB (Acid-sensing ion channel 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative MB (Acid-sensing ion channel 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of MB (Acid-sensing ion channel 2) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
