| Field | Specification |
|---|---|
| Target | |
| Alternative names | MYL2; MLC2; Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; Cardiac myosin light chain 2; Myosin light chain 2, slow skeletal/ventricular muscle isoform; MLC-2s/v; Ventricular myosin light chain 2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Myosin Light Chain 2/MLC-2V/MYL2, which shares 95.7% amino acid (aa) sequence identity with both mouse and rat Myosin Light Chain 2/MLC-2V/MYL2. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes myosin regulatory light chain 2, ventricular/cardiac muscle isoform (gene MYL2) in human, monkey, mouse and rat samples and is validated for Western blot and IHC.
MLC-2v (MYL2) is the regulatory light chain of cardiac ventricular myosin. Its phosphorylation increases contractile force and calcium sensitivity, and it supports myofibril assembly during heart development. MYL2 is a standard ventricular cardiomyocyte marker, and its mutations are linked to hypertrophic cardiomyopathy.
| Target | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (gene MYL2; UniProt P10916, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human myosin Light Chain 2/MLC-2V/MYL2. Sequence identity with the mouse and rat orthologs: 95.7%.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places myosin regulatory light chain 2, ventricular/cardiac muscle isoform in the cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in type I muscle fibers.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
