| Field | Specification |
|---|---|
| Target | |
| Alternative names | NEDD4-binding protein 1; N4BP1; Kiaa0615 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human N4BP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28N99 is a rabbit monoclonal antibody that recognizes NEDD4-binding protein 1 (gene N4bp1) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 99.1 kDa, and the supplier reports an observed band at about 110 kDa.
N4BP1 (NEDD4-binding protein 1) is a ribonuclease-domain protein that restrains cytokine and chemokine production in innate immune cells. It limits responses to TRIF-independent Toll-like receptor signals and is cleaved by caspase-8 during stronger TLR3 or TLR4 activation, releasing this brake. It is studied in inflammation and innate immune regulation.
| Target | NEDD4-binding protein 1 (gene N4bp1; UniProt Q6A037, mouse) |
|---|---|
| Clone | 28N99 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 99.1 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): PC12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human N4BP1.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places NEDD4-binding protein 1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
