| Field | Specification |
|---|---|
| Target | |
| Alternative names | N-acylethanolamine-hydrolyzing acid amidase; Acid ceramidase-like protein; Acylsphingosine deacylase NAAA; N-acylsphingosine amidohydrolase-like; ASAH-like protein; NAAA; ASAHL; PLT |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NAAA recombinant protein (Position: R35-L325). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects N-acylethanolamine-hydrolyzing acid amidase (gene NAAA) in human and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 40.1 kDa, and the supplier reports an observed band at about 38 kDa.
N-acylethanolamine acid amidase (NAAA) is a lysosomal cysteine hydrolase that degrades bioactive fatty acid ethanolamides, with a strong preference for palmitoylethanolamide (PEA). By ending PEA signaling, it influences inflammation and pain, and it is studied alongside FAAH in lipid signaling and anti-inflammatory drug research.
| Target | N-acylethanolamine-hydrolyzing acid amidase (gene NAAA; UniProt Q02083, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 40.1 kDa |
| Observed MW | 38 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells, human MCF-7 cells, rat liver tissue, rat kidney tissue, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human NAAA fragment (Arg35–Leu325), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places N-acylethanolamine-hydrolyzing acid amidase in the lysosome and membrane. Tissue expression noted by UniProt (human): Expressed in numerous tissues, with highest levels in liver and kidney, followed by pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
