| Field | Specification |
|---|---|
| Target | |
| Alternative names | NADH-ubiquinone oxidoreductase chain 2; NADH dehydrogenase subunit 2; Mtnd2; mt-Nd2; Nd2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse NADH2/Mtnd2, which shares 80% amino acid (aa) sequence identity with rat NADH2/Mtnd2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects NADH-ubiquinone oxidoreductase chain 2 (gene mt-Nd2) in mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 38.8 kDa, and the supplier reports an observed band at about 39 kDa.
MT-ND2 is a mitochondrial DNA-encoded core subunit of the membrane arm of complex I (NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain. It is needed for complex I assembly and activity, which transfers electrons from NADH to ubiquinone while pumping protons across the inner membrane.
| Target | NADH-ubiquinone oxidoreductase chain 2 (gene mt-Nd2; UniProt P03893, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 38.8 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse, Rat) |
Samples with a confirmed band (WB): rat thymus tissue, rat spleen tissue, mouse thymus tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): ANA-1 cells, NRK cells.
Immunogen
Synthetic peptide from the N-terminal region of mouse NADH2/Mtnd2. Sequence identity with the rat ortholog: 80%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places NADH-ubiquinone oxidoreductase chain 2 in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
