| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase TBK1;2.7.11.1;NF-kappa-B-activating kinase;T2K;TANK-binding kinase 1;TBK1;NAK; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NAK, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes serine/threonine-protein kinase TBK1 in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 83.6 kDa, and the supplier reports an observed band at about 100 kDa.
TBK1 is a serine/threonine kinase that drives type I interferon responses to viral and bacterial infection. Recruited by adaptors such as STING, MAVS and TRIF, it phosphorylates IRF3 and IRF7 so they can enter the nucleus and induce interferon genes, and it also regulates selective autophagy; TBK1 variants are linked to ALS and frontotemporal dementia.
| Target | Serine/threonine-protein kinase TBK1 (gene TBK1; UniProt Q9UHD2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 83.6 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat, Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
Samples with a confirmed band (WB): HeLa cells, rat testis tissue, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): rat testis tissue, human prostatic cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the C-terminal region of human NAK. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase TBK1 in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous with higher expression in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yanhong Song et al. (2023) Long noncoding RNA LTCONS6801 up-regulates TBK1 mediated antiviral innate immunity in miiuy croaker, Miichttys miiuy. Fish & Shellfish Immunology. 10.1016/j.fsi.2023.108801
