| Field | Specification |
|---|---|
| Target | |
| Alternative names | NACHT, LRR and PYD domains-containing protein 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1; NLRP3; C1orf7; CIAS1; NALP3; PYPAF1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NALP3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FBD-14 is a rabbit monoclonal antibody that recognizes NACHT, LRR and PYD domains-containing protein 3 (gene NLRP3) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 118.2 kDa, and the supplier reports an observed band at about 118 kDa.
NLRP3 is a cytosolic sensor that assembles the NLRP3 inflammasome with the adaptor ASC (PYCARD) and caspase-1 in response to a wide range of pathogen and danger signals, including potassium efflux and membrane damage. The complex drives IL-1β and IL-18 maturation and pyroptosis, making NLRP3 a central target in studies of inflammation and autoinflammatory, metabolic and neurodegenerative disease.
| Target | NACHT, LRR and PYD domains-containing protein 3 (gene NLRP3; UniProt Q96P20, human) |
|---|---|
| Clone | FBD-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 118.2 kDa |
| Observed MW | 118 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human lung carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): RAW264.7 cells.
Immunogen
Synthetic peptide based on the sequence of human NALP3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places NACHT, LRR and PYD domains-containing protein 3 in the cytoplasm, inflammasome, Golgi apparatus membrane and endoplasmic reticulum. Tissue expression noted by UniProt (human): Predominantly expressed in macrophages. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Peng Lv et al. (2021) XIST Inhibition Attenuates Calcium Oxalate Nephrocalcinosis-Induced Renal Inflammation and Oxidative Injury via the miR-223/NLRP3 Pathway. Oxidative Medicine and Cellular Longevity. 10.1155/2021/1676152
Juan Li et al. (2021) Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2021.112998
Ke Yao et al. (2022) Covalent Organic Framework (COF): A Drug and Carrier to Attenuate Retinal Ganglion Cells Death in an Acute Glaucoma Mouse Model. Polymers. 10.3390/polym14163265
Gui-Hong Zheng et al. (2019) Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice. Oxidative Medicine and Cellular Longevity. 10.1155/2019/5189819
Jingjing Yang et al. (2024) Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR-19b-3p/SOCS3/STAT3 axis mediated autophagy. IUBMB Life. 10.1002/iub.2907
Yen-Sung Lin et al. (2020) Mangiferin inhibits lipopolysaccharide-induced epithelial-mesenchymal transition (EMT) and enhances the expression of tumor suppressor gene PER1 in non-small cell lung cancer cells. Environmental Toxicology. 10.1002/tox.22943
Yanxia Huang et al. (2021) c-FLIP regulates pyroptosis in retinal neurons following oxygen-glucose deprivation/recovery via a GSDMD-mediated pathway. Annals of Anatomy-Anatomischer Anzeiger. 10.1016/j.aanat.2020.151672
Zhu Hanliang et al. (2024) Knockdown of long non-coding RNA SBF2-AS1 inhibits calcium oxalate-induced HK-2 cell injury by regulating the miR-302e/NLRP3 pathway. Urolithiasis. 10.1007/s00240-024-01606-y
