| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein NANOG;Homeobox transcription factor Nanog;hNanog;NANOG; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Nanog, different from the related mouse sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes homeobox protein NANOG in human, mouse and rat samples and is validated for flow cytometry and Western blot. The predicted molecular weight is 34.6 kDa, and the supplier reports an observed band at about 48 kDa.
NANOG is a homeodomain transcription factor that, together with OCT4 and SOX2, forms the core network maintaining pluripotency and self-renewal in embryonic stem cells. It is expressed in the inner cell mass and epiblast and blocks differentiation toward endoderm and trophectoderm. NANOG is a standard marker in stem cell and reprogramming research.
| Target | Homeobox protein NANOG (gene NANOG; UniProt Q9H9S0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 34.6 kDa |
| Observed MW | 48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): rat ovary tissue, mouse ovary tissue, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): A549 cells, PC-3 cells.
Immunogen
Synthetic peptide from the middle region of human nanog. It differs from the mouse sequence at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places homeobox protein NANOG in the nucleus. Tissue expression noted by UniProt (human): Expressed in testicular carcinoma and derived germ cell tumors (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Miao Yu et al. (2021) LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis. Journal of Oncology. 10.1155/2021/8493431
