| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein NANOG;Homeobox transcription factor Nanog;hNanog;NANOG; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Nanog |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EHD-14 is a rabbit monoclonal antibody that recognizes homeobox protein NANOG in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 34.6 kDa, and the supplier reports an observed band at about 37 kDa.
NANOG is a homeodomain transcription factor that, together with OCT4 and SOX2, forms the core network maintaining pluripotency and self-renewal in embryonic stem cells. It is expressed in the inner cell mass and epiblast and blocks differentiation toward endoderm and trophectoderm. NANOG is a standard marker in stem cell and reprogramming research.
| Target | Homeobox protein NANOG (gene NANOG; UniProt Q9H9S0, human) |
|---|---|
| Clone | EHD-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.6 kDa |
| Observed MW | 37 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): NCCIT cells.
Immunogen
Synthetic peptide based on the sequence of human nanog.
Reactivity Notes
The supplier lists reactivity with human. UniProt places homeobox protein NANOG in the nucleus. Tissue expression noted by UniProt (human): Expressed in testicular carcinoma and derived germ cell tumors (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
