| Field | Specification |
|---|---|
| Target | |
| Alternative names | CIAO3; NARFL; PRN; Cytosolic iron-sulfur assembly component 3; Cytosolic Fe-S cluster assembly factor NARFL; Iron-only hydrogenase-like protein 1; IOP1; Nuclear prelamin A recognition factor-like protein; Protein related to Narf |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NARFL/CIAO3 recombinant protein (Position: Y196-W476). Human NARFL/CIAO3 shares 88.3% and 86.8% amino acid (aa) sequence identity with mouse and rat NARFL/CIAO3, respectively. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cytosolic iron-sulfur assembly component 3 (gene CIAO3) in human samples and is validated for Western blot, flow cytometry and ELISA.
CIAO3 (also called NARFL or IOP1) belongs to the CIA machinery that builds iron-sulfur proteins in the cytosol. It helps transfer iron-sulfur clusters to cytoplasmic and nuclear proteins that need them for function, including enzymes involved in DNA replication and repair.
| Target | Cytosolic iron-sulfur assembly component 3 (gene CIAO3; UniProt Q9H6Q4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NARFL/CIAO3 fragment (Tyr196–Trp476), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 88.3% and 86.8%, respectively.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
