| Field | Specification |
|---|---|
| Target | |
| Alternative names | N-alpha-acetyltransferase 15, NatA auxiliary subunit;Gastric cancer antigen Ga19;N-terminal acetyltransferase;NMDA receptor-regulated protein 1;Protein tubedown-1;Tbdn100;NAA15;GA19, NARG1, NATH, TBDN100; |
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| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human NARG1, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
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| Reconstitution | |
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| Concentration | |
| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody recognizes N-alpha-acetyltransferase 15, NatA auxiliary subunit (gene NAA15) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 101.3 kDa, and the supplier reports an observed band at about 101 kDa.
NAA15 is the auxiliary subunit of NatA, the main N-terminal acetyltransferase complex, in which it anchors the catalytic subunit NAA10 to ribosomes. NatA acetylates the N-termini of a large fraction of human proteins, and NAA15 variants are associated with neurodevelopmental disorders and congenital heart disease.
| Target | N-alpha-acetyltransferase 15, NatA auxiliary subunit (gene NAA15; UniProt Q9BXJ9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 101.3 kDa |
| Observed MW | 101 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human 293T whole ce, human K562 whole cel, mouse brain tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human gastric cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human NARG1. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places N-alpha-acetyltransferase 15, NatA auxiliary subunit in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed at high levels in testis and in ocular endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
