| Field | Specification |
|---|---|
| Target | |
| Alternative names | Arylamine N-acetyltransferase 1; Aromatic-hydroxylamine O-acetyltransferase NAT1; Arylamide acetylase 1; Monomorphic arylamine N-acetyltransferase; N-acetyltransferase type 1; NAT-1; N-hydroxyarylamine O-acetyltransferase NAT1; NAT1; AAC1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NAT1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28N84 is a rabbit monoclonal antibody that recognizes arylamine N-acetyltransferase 1 (gene NAT1) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 33.9 kDa, and the supplier reports an observed band at about 30 kDa.
Arylamine N-acetyltransferase 1 (NAT1) transfers acetyl groups from acetyl-CoA to aromatic amines and hydrazines, detoxifying or activating drugs and carcinogens. Unlike NAT2, it is widely expressed across tissues and also acetylates endogenous substrates, and it is studied in xenobiotic metabolism and breast cancer.
| Target | Arylamine N-acetyltransferase 1 (gene NAT1; UniProt P18440, human) |
|---|---|
| Clone | 28N84 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.9 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): LnCaP cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human NAT1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places arylamine N-acetyltransferase 1 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
