| Field | Specification |
|---|---|
| Target | |
| Alternative names | RNA cytidine acetyltransferase; 18S rRNA cytosine acetyltransferase; N-acetyltransferase 10; N-acetyltransferase-like protein; hALP; NAT10; ALP; KIAA1709 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NAT10 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26N34 is a rabbit monoclonal antibody that recognizes RNA cytidine acetyltransferase (gene NAT10) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 115.7 kDa, and the supplier reports an observed band at about 116 kDa.
NAT10 is an RNA acetyltransferase that adds N4-acetylcytidine (ac4C) to 18S rRNA, tRNAs and many mRNAs. The mark supports ribosome biogenesis and can increase the stability and translation of modified mRNAs, and NAT10 is studied in RNA modification, cell growth and cancer.
| Target | RNA cytidine acetyltransferase (gene NAT10; UniProt Q9H0A0, human) |
|---|---|
| Clone | 26N34 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 115.7 kDa |
| Observed MW | 116 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:10000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, human K562 cells, monkey COS-7 cells, rat PC-12 cells, rat C6 cells, mouse RAW264.7 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human NAT10.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places RNA cytidine acetyltransferase in the nucleus and midbody. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tongmeng Jiang et al. (2018) Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels. Biomaterials. 10.1016/j.biomaterials.2018.10.015
Tengkai Wang et al. (2026) GNE-493 suppresses gastric cancer development by targeting NAT10-mediated ac4C modification of HK2. Cellular Signalling. 10.1016/j.cellsig.2026.112441
Zhenhua Song et al. (2018) Association of astragaloside IV-inhibited autophagy and mineralization in vascular smooth muscle cells with lncRNA H19 and DUSP5-mediated ERK signaling. Toxicology and Applied Pharmacology. 10.1016/j.taap.2018.12.002
