| Field | Specification |
|---|---|
| Target | |
| Alternative names | NAD; P; H-hydrate epimerase; Apolipoprotein A-I-binding protein; AI-BP; NAD(P)HX epimerase; NAXE; YjeF N-terminal domain-containing protein 1; YjeF_N1; AIBP; APOA1BP; YJEFN1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NAXE recombinant protein (Position: R46-Q288). Human NAXE shares 93% and 94.2% amino acid (aa) sequence identity with mouse and rat NAXE, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets NAD(P)H-hydrate epimerase (gene NAXE) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
NAXE, also called apolipoprotein A-I binding protein (AIBP), is an epimerase that interconverts the R and S forms of NAD(P)HX, damaged hydrated forms of NAD(P)H, enabling their repair. Its secreted form binds apoA-I and promotes cholesterol efflux, which limits angiogenesis and inflammation. NAXE deficiency causes a severe neurometabolic disorder.
| Target | NAD(P)H-hydrate epimerase (gene NAXE; UniProt Q8NCW5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human NAXE fragment (Arg46–Gln288), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93% and 94.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes NAD(P)H-hydrate epimerase as secreted and also located in the mitochondrion. Tissue expression noted by UniProt (human): Ubiquitously expressed, with highest levels in kidney, heart and liver.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
