Anti-NCAM NCAM1 Rabbit Monoclonal Antibody

Overview

Anti-NCAM NCAM1 Rabbit Monoclonal Antibody is an antibody targeting NCAM1. Common applications include WB, IHC, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AOGF-14; isotype: Rabbit IgG; reactivity: Human; observed MW: 70 kDa; calculated MW: 94574 MW.

Boster Bio Anti-NCAM NCAM1 Rabbit Monoclonal Antibody catalog # M00184-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Key elements and design rationale

• Target: NCAM1 — Neural cell adhesion molecule 1
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AOGF-14; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 70 kDa; Calculated: 94574 MW

Biological background

Protein function (datasheet): This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Cellular localization (datasheet): Isoform 1: Cell membrane; Single-pass type I membrane protein.

Tissue details (datasheet): Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. .

Research relevance and current trends

• Commonly studied in contexts related to Axonal Guidance Proteins,Cell Adhesion Proteins,Cell Type Marker,Developmental Biology,ECM Proteins,Growth and Development,Hematopoietic Progenitors,Membrane Proteins,Nervous System Development,Neural Signal Transduction,Neurology Process,Neuron Marker,Neuroscience,Organogenesis,Stem Cells.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.