| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neutrophil cytosol factor 4; NCF-4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox; p40phox; NCF4; SH3PXD4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NCF4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27N19 is a rabbit monoclonal antibody that recognizes neutrophil cytosol factor 4 (gene NCF4) in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 39.0 kDa, and the supplier reports an observed band at about 39 kDa.
p40phox (NCF4) is a regulatory subunit of the phagocyte NADPH oxidase NOX2. Its PX domain binds PI3P, targeting the oxidase to phagosome membranes so that superoxide is produced inside phagosomes. It is studied in phagocyte antimicrobial defense and inflammation.
| Target | Neutrophil cytosol factor 4 (gene NCF4; UniProt Q15080, human) |
|---|---|
| Clone | 27N19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.0 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human NCF4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places neutrophil cytosol factor 4 in the cytoplasm, endosome membrane and membrane. Tissue expression noted by UniProt (human): Expression is restricted to hematopoietic cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
