| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear receptor coactivator 4; NCoA-4; Androgen receptor coactivator 70 kDa protein; 70 kDa AR-activator; 70 kDa androgen receptor coactivator; Androgen receptor-associated protein of 70 kDa; Ferritin cargo receptor NCOA4; Ret-activating protein ELE1; NCOA4; ARA70; ELE1; RFG |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NCOA4 recombinant protein (Position: R14-M614). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects nuclear receptor coactivator 4 (gene NCOA4) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 69.7 kDa, and the supplier reports an observed band at about 70 kDa.
NCOA4 is the selective cargo receptor for ferritinophagy, the autophagic breakdown of the iron-storage protein ferritin. When cells need iron, NCOA4 delivers ferritin to lysosomes so that stored iron can be released, making it central to iron homeostasis, red blood cell production and ferroptosis research. It was first described as a coactivator of the androgen receptor.
| Target | Nuclear receptor coactivator 4 (gene NCOA4; UniProt Q13772, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 69.7 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human PC-3 cells, human HeLa cells, human HL-60 cells, human HepG2 cells, human Daudi cells, human A431 cells, rat liver tissue, rat RH35 cells, mouse liver tissue, mouse HEPA1/6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human NCOA4 fragment (Arg14–Met614), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear receptor coactivator 4 in the cytoplasmic vesicle, autolysosome, nucleus and chromosome. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Zhiqin et al. (2026) Ischemic Stroke Induces Ferroptosis and Neuroinflammation via Activation of the Microglial Tbp-Lpl Transcriptional Axis. Neurochemical Research. 10.1007/s11064-026-04812-9
Yue Zhang et al. (2024) An in-depth analysis of the effects of excessive acetochlor exposure on chicken liver health. Pesticide Biochemistry and Physiology. 10.1016/j.pestbp.2024.106280
Ting Yu et al. (2025) Isorhynchophylline protects against ferroptosis in diabetic nephropathy by activating Nrf2. Nefrologia. 10.1016/j.nefro.2025.501408
Jin Bo et al. (2026) Yishen Gushu formula exerts osteoprotective effects in OVX-Induced PMOP rats: role of ferroptosis Inhibition and iron metabolism correction. Hereditas. 10.1186/s41065-026-00642-5
