| Field | Specification |
|---|---|
| Target | |
| Alternative names | NADH-ubiquinone oxidoreductase chain 4; NADH dehydrogenase subunit 4; Mtnd4; mt-Nd4; Nd4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of rat ND4/Mtnd4, which shares 82.3% amino acid (aa) sequence identity with mouse ND4/Mtnd4. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes NADH-ubiquinone oxidoreductase chain 4 (gene Mt-nd4) in rat samples and is validated for Western blot.
MT-ND4 is a mitochondrial DNA-encoded core subunit of the membrane arm of complex I (NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain. It contributes to proton pumping and complex I activity, and the m.11778G>A mutation in MT-ND4 is the most common cause of Leber hereditary optic neuropathy.
| Target | NADH-ubiquinone oxidoreductase chain 4 (gene Mt-nd4; UniProt P05508, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Rat) |
|---|
Immunogen
Synthetic peptide from the N-terminal region of rat ND4/Mtnd4. Sequence identity with the mouse ortholog: 82.3%.
Reactivity Notes
The supplier lists reactivity with rat. UniProt places NADH-ubiquinone oxidoreductase chain 4 in the mitochondrion inner membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
