| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fatty acid-binding protein, liver; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP; Squalene- and sterol-carrier protein; SCP; Z-protein; p14; Fabp1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human NDEL1 recombinant protein (Position: K80-C320). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NDEL1 Antibody Picoband® is an antibody for NDEL1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: NDEL1 (fatty acid binding protein 1); UniProt: Q9GZM8
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 38 kDa, calculated 22105 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-NDEL1 Antibody Picoband® catalog # A02478-2.
Biological background
Biological context: Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (By similarity). Binds cholesterol (PubMed:25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).
Expression and localization notes: cellular localization: Cytoplasm., tissue context: Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta..
Common research applications
- Western blotting (WB): Compare NDEL1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of NDEL1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify NDEL1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Nuclear distribution protein nudE-like 1 is a protein that in humans is encoded by the NDEL1 gene. This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm.
- Tissue details: Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.
- Research category: Fatty Acid Oxidation,Fatty Acids,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Redox Metabolism,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
