| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear distribution protein nudE-like 1; Protein Nudel; Mitosin-associated protein 1; NDEL1; EOPA; MITAP1; NUDEL |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NDEL1 recombinant protein (Position: K80-C320). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets nuclear distribution protein nudE-like 1 (gene NDEL1) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 38.4 kDa, and the supplier reports an observed band at about 38 kDa.
NDEL1 (NudEL) is a dynein regulator closely related to NDE1. Together with LIS1 it supports dynein activity and microtubule organization at the centrosome, and it contributes to neuronal migration, neurite outgrowth and Golgi integrity. Its partnership with DISC1 has made it a subject of neurodevelopmental research.
| Target | Nuclear distribution protein nudE-like 1 (gene NDEL1; UniProt Q9GZM8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 38.4 kDa |
| Observed MW | 38 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human HEK293 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human endometrial cancer tissue, human breast cancer tissue, human gastric cancer tissue, human liver cancer tissue, human lung cancer tissue, human placenta tissue, human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Recombinant human NDEL1 fragment (Lys80–Cys320), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nuclear distribution protein nudE-like 1 in the cytoplasm and chromosome. Tissue expression noted by UniProt (human): Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
