| Field | Specification |
|---|---|
| Target | |
| Alternative names | NEDD4 family-interacting protein 2; NEDD4 WW domain-binding protein 5A; Putative MAPK-activating protein PM04/PM05/PM06/PM07; Putative NF-kappa-B-activating protein 413; NDFIP2; KIAA1165; N4WBP5A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NDFIP2 recombinant protein (Position: M16-L336). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects NEDD4 family-interacting protein 2 (gene NDFIP2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 36.4 kDa, and the supplier reports an observed band at about 39 kDa.
NDFIP2 is a membrane adaptor, related to NDFIP1, that recruits and activates NEDD4 family E3 ubiquitin ligases such as ITCH, NEDD4 and SMURF2 at endosomes and the Golgi. It thereby controls the degradation of targets including the hERG (KCNH2) potassium channel and JAK1, influencing ion channel levels and Th2 T-cell cytokine signaling.
| Target | NEDD4 family-interacting protein 2 (gene NDFIP2; UniProt Q9NV92, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 36.4 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human HepG2 cells, human SK-OV-3 cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Recombinant human NDFIP2 fragment (Met16–Leu336), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places NEDD4 family-interacting protein 2 in the endosome membrane, Golgi apparatus membrane and endosome. Tissue expression noted by UniProt (human): Expressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
