Anti-Ndufs4 Rabbit Monoclonal Antibody

Overview

This product is an anti-NDUFS4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 20N37; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, IP, Flow (as provided in the source record). Boster Bio Anti-Ndufs4 Rabbit Monoclonal Antibody catalog # M03608-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: NDUFS4 (ATP synthase subunit alpha, mitochondrial).
• Antibody format: Monoclonal; clone 20N37; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

NDUFS4 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Mitochondrial membrane ATP synthase (F (1)F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F (1). Rotation of the central stalk against the surrounding alpha (3)beta (3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity). . Reported cellular localization context: Mitochondrion inner membrane . Cell membrane; Peripheral membrane protein; Extracellular side . Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951). Tissue expression notes (as provided): Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. .

Research relevance and current trends

• Research context keywords from the source record include: ATPases,Cancer,Metabolism,Mitochondria,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Organelles,Oxidative Phosphorylation,Pathways and Processes,Plasma Membrane,Signal Transduction,Subcellular Markers,Tags & Cell Markers.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate NDUFS4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NDUFS4 expression by Western blot in cell or tissue lysates, Detect NDUFS4 in FFPE tissue sections by immunohistochemistry, Quantify NDUFS4-positive cells by flow cytometry in single-cell suspensions, Enrich NDUFS4 by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 18 kDa; calculated MW: 59751 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 18 kDa
• Cellular localization (provided): Mitochondrion inner membrane . Cell membrane; Peripheral membrane protein; Extracellular side . Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951).
• Tissue details (provided): Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.