| Field | Specification |
|---|---|
| Target | |
| Alternative names | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; Complex I-23kD; CI-23kD; NADH-ubiquinone oxidoreductase 23 kDa subunit; TYKY subunit; NDUFS8 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NDUFS8 recombinant protein (Position: M1-R210). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes NADH dehydrogenase (gene NDUFS8) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 23.7 kDa, and the supplier reports an observed band at about 23 kDa.
NDUFS8 is a core subunit of mitochondrial complex I, the enzyme that transfers electrons from NADH to ubiquinone. It carries iron-sulfur clusters in the peripheral arm of the complex and is needed for both catalytic activity and assembly.
| Target | NADH dehydrogenase (gene NDUFS8; UniProt O00217, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 23.7 kDa |
| Observed MW | 23 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human A549 cells, human MCF-7 cells, human Caco-2 cells, human PC-3 cells, human 293T cells, rat heart tissue, rat liver tissue, rat skeletal muscle tissue, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, mouse C2C12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, mouse heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HeLa cells, 293T cells.
Immunogen
Recombinant human NDUFS8 fragment (Met1–Arg210), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NADH dehydrogenase in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Expressed in all tissues with the highest level in heart and skeletal muscle and the lowest level in lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiayang Huang et al. (2026) STAT1/SLC31A1 signaling promotes diabetic retinopathy progression by mediating cuproptosis-induced M1 polarization in microglial cells. Life Sciences. 10.1016/j.lfs.2026.124241
Caihua Zhang et al. (2023) SOX18 meditates the resistance of Bmi1-expressing cells to cetuximab in HNSCC. Oral Diseases. 10.1111/odi.14596
Qiang Hu et al. (2024) The STAT1-SLC31A1 axis: Potential regulation of cuproptosis in diabetic retinopathy. Gene. 10.1016/j.gene.2024.148861
