| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nectin-3; CDw113; Nectin cell adhesion molecule 3; Poliovirus receptor-related protein 3; CD113; NECTIN3; PRR3; PVRL3 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Nectin 3/NECTIN3 recombinant protein (Position: E92-N296). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Nectin 3/NECTIN3 Antibody Picoband® is an antibody for NECTIN3 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: NECTIN3 (nectin cell adhesion molecule 3); UniProt: Q9NQS3
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 80 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Nectin 3/NECTIN3 Antibody Picoband® catalog # A09633-3.
Biological background
Biological context: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
Expression and localization notes: cellular localization: Cell membrane. Single-pass membrane protein. Postsynaptic cell membrane., tissue context: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines..
Common research applications
- Western blotting (WB): Compare NECTIN3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of NECTIN3 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify NECTIN3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Nectin-3, also known as poliovirus receptor-related 3, is a 95 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is mapped to 3q13.13. This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of ional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Single-pass membrane protein. Postsynaptic cell membrane.
- Tissue details: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
- Research category: Epigenetics and Nuclear Signaling,Mediator Complex,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
