| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Loricrin; LOR; LRN |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse Nefh recombinant protein (Position: Y109-E466). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Nefh Antibody Picoband® is an antibody for Nefh detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Nefh (loricrin); UniProt: P19246
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 117-220 kDa, calculated 39411 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Nefh Antibody Picoband® catalog # A05307.
Biological background
Biological context: Major keratinocyte cell envelope protein.
Expression and localization notes: cellular localization: Nucleoplasm. Cytoplasm., tissue context: Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels..
Common research applications
- Western blotting (WB): Compare Nefh levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Nefh in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Nefh-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleoplasm. Cytoplasm.
- Tissue details: Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
- Research category: Cell Biology,Cytokines,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,Interferons,Proteasome / Ubiquitin,Proteolysis/Ubiquitin,Ubiquitin & Ubiquitin Like Modifiers,Ubiquitin E3 Enzymes
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
