Anti-NEFM/Nf M Rabbit Monoclonal Antibody

Overview

Anti-NEFM/Nf M Rabbit Monoclonal Antibody is an antibody targeting NEFM. Common applications include WB, IHC, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AAGI-14; isotype: Rabbit IgG; reactivity: Human; observed MW: 94 kDa; calculated MW: 102472 MW.

Boster Bio Anti-NEFM/Nf M Rabbit Monoclonal Antibody catalog # M06821. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Key elements and design rationale

• Target: NEFM — Neurofilament medium polypeptide
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AAGI-14; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 94 kDa; Calculated: 102472 MW

Biological background

Protein function (datasheet): Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Cellular localization (datasheet): Nucleus. Chromosome.

Tissue details (datasheet): Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate. .

Research relevance and current trends

• Commonly studied in contexts related to Alzheimer's Disease,Cell Adhesion Proteins,Cell Type Marker,Cytoskeleton,Cytoskeleton/ECM,Developmental Biology,Intermediate Filaments,Lineage Markers,Lineage Specification,Neurodegenerative Disease,Neurology Process,Neuron Marker,Neuroscience,Signal Transduction,Stem Cells.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.