| Field | Specification |
|---|---|
| Target | |
| Alternative names | NEK11; Serine/threonine-protein kinase Nek11; EC 2.7.11.1; Never in mitosis A-related kinase 11; NimA-related protein kinase 11 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NEK11 recombinant protein (Position: E72-Q630). Human NEK11 shares 71.1% amino acid (aa) sequence identity with mouse NEK11. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes serine/threonine-protein kinase Nek11 in human, mouse and rat samples and is validated for Western blot and ELISA.
NEK11 is a serine/threonine kinase of the NIMA-related kinase (NEK) family. It acts in the G2/M checkpoint after DNA damage, where it phosphorylates the phosphatase CDC25A to mark it for degradation, helping to hold cells back from entering mitosis.
| Target | Serine/threonine-protein kinase Nek11 (gene NEK11; UniProt Q8NG66, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL (Human) |
Immunogen
Recombinant human NEK11 fragment (Glu72–Gln630), expressed in E. coli. Sequence identity with the mouse ortholog: 71.1%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase Nek11 in the nucleus. Tissue expression noted by UniProt (human): Poorly expressed in cerebellum, trachea, lung, appendix, and uterus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
