| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase Nek7; Never in mitosis A-related kinase 7; NimA-related protein kinase 7; NEK7 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NEK7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22N57 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase Nek7 in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 34.6 kDa, and the supplier reports an observed band at about 35 kDa.
NEK7 is a small NIMA-related kinase closely related to NEK6. It supports centrosome microtubule nucleation, spindle formation and cytokinesis, and it also serves, independently of its kinase activity, as an essential partner for activation of the NLRP3 inflammasome.
| Target | Serine/threonine-protein kinase Nek7 (gene NEK7; UniProt Q8TDX7, human) |
|---|---|
| Clone | 22N57 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.6 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human NEK7.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase Nek7 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
