| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nesprin-3; KASH domain-containing protein 3; KASH3; Nuclear envelope spectrin repeat protein 3; SYNE3; C14orf139; C14orf49; LINC00341 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Nesprin3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30S39 is a rabbit monoclonal antibody that recognizes nesprin-3 (gene SYNE3) in human samples and is validated for Western blot. The predicted molecular weight is 112.2 kDa, and the supplier reports an observed band at about 100 kDa.
Nesprin-3 (SYNE3) is a KASH-domain protein of the outer nuclear membrane that joins SUN proteins in the LINC complex. Through binding to plectin it links the nucleus to the intermediate filament network, and it is studied for its roles in nuclear anchoring, endothelial cell shape and polarized migration under fluid flow.
| Target | Nesprin-3 (gene SYNE3; UniProt Q6ZMZ3, human) |
|---|---|
| Clone | 30S39 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 112.2 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): 293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human nesprin3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nesprin-3 in the nucleus outer membrane, nucleus envelope and rough endoplasmic reticulum. Tissue expression noted by UniProt (human): Expressed in aortic endothelial cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
