| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nestin;NES;Nbla00170; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Nestin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FBH-14 is a rabbit monoclonal antibody that recognizes nestin in human, mouse, rat and pig samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 177.4 kDa, and the supplier reports an observed band at about 270–300 kDa.
Nestin (NES) is a class VI intermediate filament protein expressed mainly in dividing neural progenitor cells and other stem or progenitor cells. It helps reorganize vimentin filaments during mitosis and is required for progenitor survival and self-renewal, and it is one of the most widely used markers of neural stem and progenitor cells.
| Target | Nestin (gene NES; UniProt P48681, human) |
|---|---|
| Clone | FBH-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat, Pig |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 177.4 kDa |
| Observed MW | 270–300 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human U251 cells, human SH-SY5Y cells, rat C6 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human diffuse large B-cells lymphoma of the intestine tissue, human glioma tissue, human non-small cells lung cancer tissue, mouse brain tissue, rat brain tissue, pig brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human brain tissue, rat brain tissue.
Immunogen
Synthetic peptide based on the sequence of human nestin.
Reactivity Notes
The supplier lists reactivity with human, mouse, rat and pig. Tissue expression noted by UniProt (human): CNS stem cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Xiaoting Meng et al. (2023) Electrical stimulation induced structural 3D human engineered neural tissue with well-developed neuronal network and functional connectivity. Journal of Neural Engineering. 10.1088/1741-2552/ace658
Lei Zhang et al. (2019) Microvesicles Derived from Human Embryonic Neural Stem Cells Inhibit the Apoptosis of HL-1 Cardiomyocytes by Promoting Autophagy and Regulating AKT and mTOR via Transporting HSP-70. Stem Cells International. 10.1155/2019/6452684
Rui Wei et al. (2013) Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies. PLoS One. 10.1371/journal.pone.0072513
Demin Lv et al. (2024) LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts. Molecular Medicine Reports. 10.3892/mmr.2024.13365
JUAN LI et al. (2023) Improved Cell Properties of Human Dental Pulp Stem Cells (hDPSCs) Isolated and Expanded in a GMP Compliant and Xenogeneic Serum-free Medium. In Vivo. 10.21873/invivo.13364
Junfeng Liu et al. (2018) Radiation-induced G2/M arrest rarely occurred in glioblastoma stem-like cells. International Journal of Radiation Biology. 10.1080/09553002.2018.1440094
Fan-Wei Meng et al. (2020) Formation of new lymphatic vessels in glioma: An immunohistochemical analysis. Neuropathology. 10.1111/neup.12625
