| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-NETO1 (extracellular) Antibody is an antibody targeting Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IF, IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1 (also reported as Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1).
- Immunogen/epitope region: Extracellular, N-terminus.
- Homology note: Rat - identical; human - 12/14 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
NETO proteins are auxiliary subunits that play crucial roles in modulating the biophysical properties of Kainate receptors (KARs) and N-methyl-D-aspartate receptors (NMDARs). NETO proteins modulate these receptors both in vivo and in vitro. NETO1 is also responsible for the slow kinetics of postsynaptic KAR-mediated currents and regulate synaptic recruitment of Kainate receptors1.NETO1 is a single transmembrane domain protein containing two extracellular CUB domains of about 110 amino acids, a low-density lipoprotein receptor domain, and a cytoplasmic tail of almost 167 amino acids that includes a C-terminal TRV class I PDZ binding motif2.NETO1 and NETO2, are highly homologous, but have a different expression patterns and distinct functional effects.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-GC045.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-GC045; Negative control: BLP-GC045.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
