Anti-Neuronatin Rabbit Monoclonal Antibody

SKU:BHA21009779
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Boster Bio
Boster Bio
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Overview
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Anti-NNAT antibody (Rabbit monoclonal, clone 22N82, IgG). Validated for Western blot. Reactivity: Human, Mouse. Recognizes neuronatin. Observed band in Western blot: about 9 kDa. Supplied in liquid form, ready for use in standard immunodetection workflows.
Target NNAT
clone number 22N82
Host Rabbit
Reactivity Human,Mouse
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M08311 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: WB 1:500-2000<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target NNAT
Alternative names Neuronatin; NNAT
UniProt # Q16517
Host Rabbit
Clone 22N82
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human Neuronatin
Molecular weight 9.2 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Mitochondrion inner membrane ; Single-pass membrane protein .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M08311
Main SKU BHA21009779
Rabbit Monoclonal

Product Overview

Clone 22N82 is a rabbit monoclonal antibody that recognizes neuronatin (gene NNAT) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 9.2 kDa, and the supplier reports an observed band at about 9 kDa.

Neuronatin (NNAT) is a small membrane proteolipid of the endoplasmic reticulum encoded by an imprinted, paternally expressed gene. It is abundant in the developing brain and pituitary and may act as a regulatory subunit of ion channels. It is studied in brain development, imprinting and metabolic regulation, including insulin secretion.

Target Neuronatin (gene NNAT; UniProt Q16517, human)
Clone 22N82
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 9.2 kDa
Observed MW 9 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): SH-SY5Y cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human neuronatin.

Reactivity Notes

The supplier lists reactivity with human and mouse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 9.2 kDa; observed band at about 9 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human neuronatin.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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