| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neuropilin-1;Vascular endothelial cell growth factor 165 receptor;CD304;NRP1;NRP, VEGF165R; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes neuropilin-1 (gene NRP1) in human and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 103.1 kDa, and the supplier reports an observed band at about 103 kDa.
Neuropilin-1 (NRP1) is a transmembrane co-receptor for class 3 semaphorins and for VEGF-A isoforms such as VEGF165. It shapes axon guidance, blood vessel formation and heart development, and it is expressed on regulatory T cells. NRP1 also binds C-end rule peptide motifs and is studied in angiogenesis, neural development, immunity and cancer.
| Target | Neuropilin-1 (gene NRP1; UniProt O14786, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 103.1 kDa |
| Observed MW | 103 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): U87 cells, A549 cells, human placenta tissue, rat cardiac muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant human Neuropilin 1 fragment (Lys504–Thr827), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95% and 94%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. Tissue expression noted by UniProt (human): The expression of isoforms 1 and 2 does not seem to overlap. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.