| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neuropilin-1;Vascular endothelial cell growth factor 165 receptor;CD304;NRP1;NRP, VEGF165R; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Neuropilin 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BGO-14 is a rabbit monoclonal antibody that recognizes neuropilin-1 (gene NRP1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 103.1 kDa, and the supplier reports an observed band at about 120 kDa.
Neuropilin-1 (NRP1) is a transmembrane co-receptor for class 3 semaphorins and for VEGF-A isoforms such as VEGF165. It shapes axon guidance, blood vessel formation and heart development, and it is expressed on regulatory T cells. NRP1 also binds C-end rule peptide motifs and is studied in angiogenesis, neural development, immunity and cancer.
| Target | Neuropilin-1 (gene NRP1; UniProt O14786, human) |
|---|---|
| Clone | BGO-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 103.1 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human PC-3 cells, human A549 cells, human U251 cells, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human stomach cancer tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human neuropilin 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): The expression of isoforms 1 and 2 does not seem to overlap. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yayuan Liu et al. (2016) Dual Receptor Recognizing Cell Penetrating Peptide for Selective Targeting, Efficient Intratumoral Diffusion and Synthesized Anti-Glioma Therapy. Theranostics. 10.7150/thno.13532
Sheng Yin et al. (2018) Dual receptor recognizing liposomes containing paclitaxel and hydroxychloroquine for primary and metastatic melanoma treatment via autophagy-dependent and independent pathways. Journal of Controlled Release. 10.1016/j.jconrel.2018.08.015
Yashi Wang et al. (2020) A dual receptors-targeting and size-switchable “cluster bomb” co-loading chemotherapeutic and transient receptor potential ankyrin 1 (TRPA-1) inhibitor for treatment of triple negative breast cancer. Journal of Controlled Release. 10.1016/j.jconrel.2020.02.010
Hu Liehai et al. (2025) Developmental exposure to methyl ester sulfonate induces autism-like behavioral deficits in mice by dysregulation of the Notch/Hes signaling pathway disrupting neuronal differentiation. Archives of Toxicology. 10.1007/s00204-025-04157-2
Wang Xue Jun et al. (2026) Interaction between CD82 and integrin αVβ3 selectively regulates collective movement of tumor cells via endolysosomal trafficking. Cellular and Molecular Life Sciences. 10.1007/s00018-026-06303-0
Ainara González-Moro et al. (2025) Sex-specific molecular hallmarks point to increased atherogenesis susceptibility in male senescence-accelerated mice. Life Sciences. 10.1016/j.lfs.2025.123529
Yan Huang et al. (2019) Shear Stress Promotes Arterial Endothelium-Oriented Differentiation of Mouse-Induced Pluripotent Stem Cells. Stem Cells International. 10.1155/2019/1847098
