| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neutrophil elastase;3.4.21.37;Elastase-2;Leukocyte elastase;Elane;Ela2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265). Mouse Elastase/ELANE/ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase/ELANE/ELA2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets neutrophil elastase (gene Elane) in mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 28.6 kDa, and the supplier reports an observed band at about 29 kDa.
Neutrophil elastase (ELANE) is a serine protease stored in the azurophil granules of neutrophils. It degrades bacterial proteins and extracellular matrix components such as elastin, and it is studied in host defense, lung injury and inflammatory disease. ELANE mutations cause severe congenital and cyclic neutropenia.
| Target | Neutrophil elastase (gene Elane; UniProt Q3UP87, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 28.6 kDa |
| Observed MW | 29 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 13% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Elastase/ELANE/ELA2 fragment (Ser27–Asn265), expressed in E. coli. Sequence identity with the rat ortholog: 92%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places neutrophil elastase in the cytoplasmic vesicle, lysosome, cytolytic granule and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Heng et al. (2024) Highly sensitive magnetic particle imaging of abdominal aortic aneurysm NETosis with anti-Ly6G iron oxide nanoparticles. Cell Death Discovery. 10.1038/s41420-024-02156-3
Heng Wang et al. (2023) Neutrophil Extracellular Traps Aggravate Contrast-Induced Acute Kidney Injury by Damaging Glomeruli and Peritubular Capillaries. Journal of Inflammation Research. 10.2147/JIR.S433110
Zhang Yanxia et al. (2019) Association of TREM-1, IL-1β, IL-33/ST2, and TLR Expressions With the Pathogenesis of Ocular Toxoplasmosis in Mouse Models on Different Genetic Backgrounds. Frontiers in Microbiology. 10.3389/fmicb.2019.02264
Abdelnabi Mohamed N et al. (2026) IL-17A-induced NETs are profibrogenic in mice with metabolic dysfunction-associated steatotic liver disease (MASLD). Journal of Immunology. 10.1093/jimmun/vkaf332
Mei Xu et al. (2020) Trichinella spiralis co-infection exacerbates Plasmodium berghei malaria-induced hepatopathy. Parasites & Vectors. 10.1186/s13071-020-04309-6
