| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neutrophil elastase;3.4.21.37;Bone marrow serine protease;Elastase-2;Human leukocyte elastase;HLE;Medullasin;PMN elastase;ELANE;ELA2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Neutrophil Elastase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOCI-5 is a rabbit monoclonal antibody that recognizes neutrophil elastase (gene ELANE) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 28.5 kDa, and the supplier reports an observed band at about 29 kDa.
Neutrophil elastase (ELANE) is a serine protease stored in the azurophil granules of neutrophils. It degrades bacterial proteins and extracellular matrix components such as elastin, and it is studied in host defense, lung injury and inflammatory disease. ELANE mutations cause severe congenital and cyclic neutropenia.
| Target | Neutrophil elastase (gene ELANE; UniProt P08246, human) |
|---|---|
| Clone | AOCI-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 28.5 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:30 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human neutrophil Elastase.
Reactivity Notes
The supplier lists reactivity with human. UniProt places neutrophil elastase in the cytoplasmic vesicle, lysosome, cytolytic granule and nucleus. Tissue expression noted by UniProt (human): Bone marrow cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaolin Ma et al. (2025) Paeonol inhibits NETs-mediated foam cell inflammation through the CitH3/NLRP3/caspase-1 signaling pathway in atherosclerosis. International Immunopharmacology. 10.1016/j.intimp.2025.114340
Min Hu et al. (2022) NETs promote ROS production to induce human amniotic epithelial cell apoptosis via ERK1/2 signaling in spontaneous preterm birth. American Journal of Reproductive Immunology. 10.1111/aji.13656
