| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear factor NF-kappa-B p105 subunit;DNA-binding factor KBF1;EBP-1;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1;Nuclear factor NF-kappa-B p50 subunit;NFKB1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NF-κB (p105/p50) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ID-14 is a rabbit monoclonal antibody that recognizes nuclear factor NF-kappa-B p105 subunit (gene NFKB1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 105.4 kDa; the supplier reports observed bands at 50, 105 kDa.
NFKB1 encodes p105, a precursor that is processed into the p50 subunit of NF-κB. The p50 subunit pairs with RelA (p65) or other Rel proteins to bind κB sites in DNA, while unprocessed p105 acts as an IκB-like inhibitor, making NFKB1 central to inflammation, immunity and cell survival signaling.
| Target | Nuclear factor NF-kappa-B p105 subunit (gene NFKB1; UniProt P19838, human) |
|---|---|
| Clone | ID-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 105.4 kDa |
| Observed MW | 50, 105 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human RT4 cells, human A431 cells, human SiHa cells, human Caco-2 cells, rat PC-12 cells, rat NRK cells, mouse NIH/3T3 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human NF-κB (p105/p50).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear factor NF-kappa-B p105 subunit in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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