| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10; NFKB2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NFkB p100 / p52 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18N03 is a rabbit monoclonal antibody that recognizes nuclear factor NF-kappa-B p100 subunit (gene NFKB2) in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 96.7 kDa, and the supplier reports an observed band at about 110 kDa.
NFKB2 encodes p100, a precursor that is processed to the p52 subunit of NF-κB. Processing of p100 to p52 is the hallmark of the non-canonical NF-κB pathway, which is triggered by receptors such as BAFF-R, CD40 and LTβR and is important for lymphoid organ development and B-cell maturation. Antibodies that detect both forms help follow this processing step.
| Target | Nuclear factor NF-kappa-B p100 subunit (gene NFKB2; UniProt Q00653, human) |
|---|---|
| Clone | 18N03 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 96.7 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human Jurkat cells, human A549 cells, human Caco-2 cells, human U251 cells, rat heart tissue, rat PC-12 cells, mouse heart tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human NFkB p100 / p52.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear factor NF-kappa-B p100 subunit in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mengyuan Li et al. (2023) NLRP12 attenuates ozone-induced pulmonary inflammation by regulating canonical NF-κB Pathway. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2023.115275
Haiwen Zhang et al. (2021) Vitamin D3 Protects Mice from Diquat-Induced Oxidative Stress through the NF-κB/Nrf2/HO-1 Signaling Pathway. Oxidative Medicine and Cellular Longevity. 10.1155/2021/6776956
Wei Wang et al. (2024) Sulforaphane inhibits the migration and invasion of BPDE-induced lung adenocarcinoma cells by regulating NLRP12. Toxicology and Applied Pharmacology. 10.1016/j.taap.2024.116916
