| Field | Specification |
|---|---|
| Target | |
| Alternative names | NF-kappa-B inhibitor zeta; I-kappa-B-zeta; IkB-zeta; IkappaBzeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; Molecule possessing ankyrin repeats induced by lipopolysaccharide; MAIL; NFKBIZ; IKBZ |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NFKBIZ recombinant protein (Position: Q247-H577). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes NF-kappa-B inhibitor zeta (gene NFKBIZ) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 78.1 kDa, and the supplier reports an observed band at about 85 kDa.
IκBζ (NFKBIZ) is an atypical nuclear member of the IκB family that is induced by IL-1 and Toll-like receptor signaling. Rather than holding NF-κB in the cytoplasm, it acts in the nucleus to either inhibit or promote transcription of selected NF-κB targets such as LCN2 and IL-6.
| Target | NF-kappa-B inhibitor zeta (gene NFKBIZ; UniProt Q9BYH8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 78.1 kDa |
| Observed MW | 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human RT4 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): SIHA cells.
Samples with a confirmed signal (flow cytometry): HEL cells.
Immunogen
Recombinant human NFKBIZ fragment (Gln247–His577), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places NF-kappa-B inhibitor zeta in the nucleus. Tissue expression noted by UniProt (human): Expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
