| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ephexin-1; Eph-interacting exchange protein; Neuronal guanine nucleotide exchange factor; NGEF |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NGEF recombinant protein (Position: E82-Q627). Human NGEF shares 91.1% and 90.9% amino acid (aa) sequence identity with mouse and rat NGEF, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ephexin-1 (gene NGEF) in human samples and is validated for Western blot, flow cytometry and ELISA.
Ephexin-1 (NGEF) is a Dbl-family guanine nucleotide exchange factor for Rho GTPases, highly expressed in the brain. Downstream of the EphA4 receptor, it shifts activity toward RHOA, which drives growth cone collapse during axon guidance and regulates dendritic spine development.
| Target | Ephexin-1 (gene NGEF; UniProt Q8N5V2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NGEF fragment (Glu82–Gln627), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91.1% and 90.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ephexin-1 in the cytoplasm, membrane and cell projection. Tissue expression noted by UniProt (human): Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
