| Field | Specification |
|---|---|
| Target | |
| Alternative names | Reticulon-4 receptor-like 1; Nogo receptor-like 2; Nogo-66 receptor homolog 2; Nogo-66 receptor-related protein 3; NgR3; RTN4RL1; NGRH2; NGRL2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NgR3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29R59 is a rabbit monoclonal antibody that recognizes reticulon-4 receptor-like 1 (gene RTN4RL1) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 49.1 kDa, and the supplier reports an observed band at about 49 kDa.
NgR3 (RTN4RL1) is a member of the Nogo receptor family expressed on neurons. It binds chondroitin sulfate proteoglycans and heparin, activates Rho signaling to limit neurite outgrowth, and shares partly overlapping roles with NgR1 in brain development, axon regeneration and the control of dendritic spine and synapse numbers.
| Target | Reticulon-4 receptor-like 1 (gene RTN4RL1; UniProt Q86UN2, human) |
|---|---|
| Clone | 29R59 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 49.1 kDa |
| Observed MW | 49 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ngR3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places reticulon-4 receptor-like 1 in the cell membrane, membrane raft, perikaryon and cell projection. Tissue expression noted by UniProt (human): Predominantly expressed in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
