| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nicastrin; NCSTN; KIAA0253 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Nicastrin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19N33 is a rabbit monoclonal antibody that recognizes nicastrin (gene NCSTN) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 78.4 kDa; the supplier reports observed bands at 120, 140 kDa.
Nicastrin (NCSTN) is a single-pass membrane glycoprotein and essential subunit of γ-secretase, together with presenilin, APH-1 and PEN-2. This protease cuts substrates such as Notch receptors and the amyloid precursor protein (APP) within the membrane, so nicastrin is studied in Notch signaling, Alzheimer′s disease research and skin biology.
| Target | Nicastrin (gene NCSTN; UniProt Q92542, human) |
|---|---|
| Clone | 19N33 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 78.4 kDa |
| Observed MW | 120, 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human nicastrin.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nicastrin in the membrane, cytoplasmic vesicle membrane and melanosome. Tissue expression noted by UniProt (human): Detected in brain (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
