| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neuronal acetylcholine receptor subunit alpha-3; CHRNA3; NACHRA3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CHRNA3, which shares 97.4% and 94.7% amino acid (aa) sequence identity with mouse and rat CHRNA3, respectively. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets neuronal acetylcholine receptor subunit alpha-3 (gene CHRNA3) in human, mouse and rat samples and is validated for flow cytometry and Western blot. The predicted molecular weight is 57.5 kDa, and the supplier reports an observed band at about 50–55 kDa.
The neuronal acetylcholine receptor alpha-3 subunit (CHRNA3) forms part of pentameric nicotinic acetylcholine receptors, ligand-gated cation channels. Alpha-3-containing receptors are the main type in autonomic ganglia, and the CHRNA5-CHRNA3-CHRNB4 gene cluster is studied in nicotine dependence.
| Target | Neuronal acetylcholine receptor subunit alpha-3 (gene CHRNA3; UniProt P32297, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 57.5 kDa |
| Observed MW | 50–55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the C-terminal region of human CHRNA3. Sequence identity with the mouse and rat orthologs: 97.4% and 94.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neuronal acetylcholine receptor subunit alpha-3 in the synaptic cell membrane, cell membrane, endoplasmic reticulum and Golgi apparatus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yiqiao Liu et al. (2019) Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets. Marine Drugs. 10.3390/md17050256
