| Field | Specification |
|---|---|
| Target | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Nir2/PITPNM1 recombinant protein (Position: E418-R831). Human PITPNM1 shares 88.9% and 89.9% amino acid (aa) sequence identity with mouse and rat PITPNM1, respectively. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets membrane-associated phosphatidylinositol transfer protein 1 (gene PITPNM1) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
PITPNM1 (Nir2) is a membrane-associated phosphatidylinositol transfer protein that works at endoplasmic reticulum-plasma membrane contact sites. It moves phosphatidylinositol to the plasma membrane and phosphatidic acid back to the ER, replenishing PI(4,5)P2 after receptor-triggered phospholipase C activity.
| Target | Membrane-associated phosphatidylinositol transfer protein 1 (gene PITPNM1; UniProt O00562, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human Nir2/PITPNM1 fragment (Glu418–Arg831), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 88.9% and 89.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places membrane-associated phosphatidylinositol transfer protein 1 in the cell membrane, Golgi apparatus and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
