| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nitric oxide synthase, brain;1.14.13.39;BNOS;Constitutive NOS;NC-NOS;N |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant neuronal NOS fragment (amino acids 1-181) from rat brain. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1) is an antibody targeting NOS1. Common applications include WB. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: NOS-B1; isotype: Mouse IgG1; reactivity: Goat,Human,Pig,Rat; observed MW: 200 kDa; calculated MW: 160559 MW.
Boster Bio Anti-Nitric Oxide Synthase, Brain (1-181) NOS1 Antibody (Monoclonal, NOS-B1) catalog # MA1072. Tested in WB applications. This antibody reacts with Goat, Human, Pig, Rat.
Key elements and design rationale
- Target: NOS1 — Nitric oxide synthase, brain
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: NOS-B1; Isotype: Mouse IgG1
- Species reactivity: Goat,Human,Pig,Rat
- Molecular weight guidance: Observed: 200 kDa; Calculated: 160559 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non- adrenergic and non-cholinergic nerves in the colorectum.
Scientific background (datasheet): Nitric Oxide Synthase 1 (NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.
Cellular localization (datasheet): Cell membrane, sarcolemma ; Peripheral membrane protein . Cell projection, dendritic spine . In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (By similarity). In neurons, enriched in dendritic spines. .
Tissue details (datasheet): Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum. .
Sequence similarities (datasheet): Belongs to the NOS family.
Research relevance and current trends
- Commonly studied in contexts related to Alzheimer's Disease,Cell Adhesion Proteins,Cell Type Marker,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Neurodegenerative Disease,Neurology Process,Neuron Marker,Neuroscience,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
