Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)

SKU:BHA21006543
Research Validated
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Overview
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Anti-NOS1 antibody (Mouse monoclonal, clone NOS-B1, Mouse IgG1). Validated for Western blot. Reactivity: Goat, Human, Pig, Rat. Recognizes nitric oxide synthase 1. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target NOS1
Clone number NOS-B1
Host Mouse
Reactivity Goat,Human,Pig,Rat
Isotype Mouse IgG1
Application(s) WB
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Options selector
Catalog no. Size Conjugation
MA1072 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated
      Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
      Form: Lyophilized
      Applications: WB
      Application details: Western blot, 0.5μg/ml, Human, goat, pig, rat
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target NOS1
Alternative names Nitric oxide synthase, brain;1.14.13.39;BNOS;Constitutive NOS;NC-NOS;N
Gene ID Human(4842), Mouse(18125), Rat(24598)
UniProt # P29476
Host Mouse
Clone NOS-B1
Clonality
  • Monoclonal
Isotype
  • Mouse IgG1
Reactivity
  • Goat
  • Human
  • Pig
  • Rat
Applications
  • Western Blot
Immunogen Recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.
Purification Ascites
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Cellular localization Cell membrane, sarcolemma ; Peripheral membrane protein . Cell projection, dendritic spine . In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (By similarity). In neurons, enriched in dendritic spines. .
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) MA1072
Main SKU BHA21006543
Mouse Monoclonal

Product Overview

Clone NOS-B1 is a mouse monoclonal antibody that recognizes nitric oxide synthase 1 (gene Nos1) in goat, human, pig and rat samples and is validated for Western blot.

Neuronal nitric oxide synthase (nNOS, NOS1) is a Ca2+/calmodulin-dependent enzyme that converts L-arginine to nitric oxide. In the brain and peripheral nerves, nitric oxide acts as an unconventional neurotransmitter, and nNOS is also expressed in skeletal muscle. It is studied in synaptic plasticity, neurotoxicity and neuromuscular physiology, and it can promote S-nitrosylation of target proteins.

Target Nitric oxide synthase 1 (gene Nos1; UniProt P29476, rat)
Clone NOS-B1
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG1
Reactivity Goat, Human, Pig, Rat
Purification Ascites
Form Lyophilized
Formulation 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers
Reconstitution 100 µg/mL after adding 1 mL PBS
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot Not specified by the supplier

Immunogen

Recombinant neuronal NOS fragment (amino acids 1–181) from rat brain.

Reactivity Notes

The supplier lists reactivity with goat, human, pig and rat. UniProt places nitric oxide synthase 1 in the cell membrane and cell projection. Tissue expression noted by UniProt (rat): Isoform N-NOS-1 is expressed in brain and colorectum.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for?
A.Validated applications: Western blot. Starting dilutions are not specified by the supplier; titrate for each application.
Q.Which species does this antibody react with?
A.Validated reactivity: Goat, Human, Pig, Rat. Other species are not listed by the supplier.
Q.Has this antibody been tested on canine samples?
A.Reactivity with canine samples has not been tested by the supplier. Aligning the immunogen sequence with the canine ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What immunogen was used to raise this antibody?
A.Recombinant neuronal NOS fragment (amino acids 1–181) from rat brain.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Yan-Min Chen et al. (2002) Distribution of constitutive nitric oxide synthase in the jejunum of adult rat. World Journal of Gastroenterology. 10.3748/wjg.v8.i3.537

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