| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Homeobox protein Nkx-2.5;Cardiac-specific homeobox;Homeobox protein CSX;Homeobox protein NK-2 homolog E;NKX2-5;CSX, NKX2.5, NKX2E; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Nkx2.5, different from the related mouse and rat sequences by five amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of NKX2-5 (Homeobox protein Nkx-2.5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Nkx2.5/NKX2-5 Antibody Picoband® catalog # PB9293. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human Nkx2.5, different from the related mouse and rat sequences by five amino acids.
- Molecular weight context: reported MW: 35 kDa; calculated MW: 34918 MW
- Reactivity: Human,Mouse
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Homeobox protein Nkx-2.5; Homeobox protein Nkx-2.5. Homeobox protein Nkx-2.5, also known as NKX2E or CSX is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2.5 and Tbx5 ly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. The cardiac homeobox protein Nkx2.5 is essential in cardiac development, and mutations in CSX (which encodes Nkx2.5) cause various congenital heart malformations. Functional note: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development. . Reported localization: Nucleus . Expression/tissue context: Expressed only in the heart.
Research relevance and current trends
- Cardiogenesis: Researchers commonly examine how NKX2-5 (Homeobox protein Nkx-2.5) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how NKX2-5 (Homeobox protein Nkx-2.5) relates to this theme using model systems and orthogonal readouts.
- Developmental Biology: Researchers commonly examine how NKX2-5 (Homeobox protein Nkx-2.5) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative NKX2-5 (Homeobox protein Nkx-2.5) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the NK-2 homeobox family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
