| Field | Specification |
|---|---|
| Target | |
| Alternative names | NLE1; HUSSY-07; Notchless protein homolog 1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NLE1 recombinant protein (Position: M1-R385). Human NLE1 shares 94.3% amino acid (aa) sequence identity with mouse NLE1. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets notchless protein homolog 1 (gene NLE1) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
Notchless homolog 1 (NLE1) is a WD-repeat protein that modulates Notch signaling and influences expression of CDKN1A and several Wnt pathway genes. It is also involved in ribosome biogenesis, and mouse studies show it is required for survival of the inner cell mass in the early embryo.
| Target | Notchless protein homolog 1 (gene NLE1; UniProt Q9NVX2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NLE1 fragment (Met1–Arg385), expressed in E. coli. Sequence identity with the mouse ortholog: 94.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places notchless protein homolog 1 in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
