| Field | Specification |
|---|---|
| Target | |
| Alternative names | NACHT, LRR and PYD domains-containing protein 9; Nucleotide-binding oligomerization domain protein 6; PYRIN and NACHT-containing protein 12; NLRP9; NALP9; NOD6; PAN12 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NLRP9 recombinant protein (Position: H118-H746). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes NACHT, LRR and PYD domains-containing protein 9 (gene NLRP9) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 113.3 kDa, and the supplier reports an observed band at about 113 kDa.
NLRP9 is a NOD-like receptor that forms an inflammasome with ASC (PYCARD) and caspase-1. In intestinal epithelial cells it senses rotavirus RNA with the help of DHX9, leading to IL-18 release and pyroptosis. It is also expressed in oocytes and is studied in intestinal antiviral immunity and early development.
| Target | NACHT, LRR and PYD domains-containing protein 9 (gene NLRP9; UniProt Q7RTR0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 113.3 kDa |
| Observed MW | 113 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human NLRP9 fragment (His118–His746), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places NACHT, LRR and PYD domains-containing protein 9 in the cytoplasm and inflammasome. Tissue expression noted by UniProt (human): Expressed in ileum intestinal epithelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
