| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleoside diphosphate kinase A; NDP kinase A; NDPK A; Farnesyl diphosphate kinase NME1; Histidine protein kinase NME1; Metastasis inhibition factor nm23; NM23-H1; Non-specific serine/threonine protein kinase NME1; Nucleoside diphoshate kinase 1; NDK1; Putative 3'-5'-DNA exonuclease NDK1; Putative granzyme A-activated DNA endonuclease; GAAD; Tumor metastatic process-associated protein; NME1; NDPKA; NM23 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NM23 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20N83 is a rabbit monoclonal antibody that recognizes nucleoside diphosphate kinase A (gene NME1) in human samples and is validated for Western blot. The predicted molecular weight is 17.1 kDa, and the supplier reports an observed band at about 17 kDa.
NME1 (NM23-H1) is nucleoside diphosphate kinase A, which moves the terminal phosphate from ATP onto other nucleoside diphosphates via a phosphohistidine intermediate to keep NTP pools balanced. It also has histidine kinase and nuclease-related activities and was the first described metastasis suppressor, so it is studied in nucleotide metabolism and cancer metastasis.
| Target | Nucleoside diphosphate kinase A (gene NME1; UniProt P15531, human) |
|---|---|
| Clone | 20N83 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 17.1 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human NM23.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nucleoside diphosphate kinase A in the cytoplasm, nucleus and cell membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Zhu et al. (2005) Mesenchymal stem cells derived from bone marrow favor tumor cell growth in vivo. Experimental and Molecular Pathology. 10.1016/j.yexmp.2005.07.004
Runqiu Jiang et al. (2006) Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells. Oncology Reports. 10.3892/or.16.5.1021
Xiaodong Song et al. (2012) Changes in cell ultrastructure and inhibition of JAK1/STAT3 signaling pathway in CBRH-7919 cells with astaxanthin. Toxicology Mechanisms and Methods. 10.3109/15376516.2012.717119
