| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nicotinamide N-methyltransferase; NNMT |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NNMT recombinant protein (Position: K8-L264). Human NNMT shares 85.1% amino acid (aa) sequence identity with mouse NNMT. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects nicotinamide N-methyltransferase (gene NNMT) in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and ELISA.
Nicotinamide N-methyltransferase (NNMT) transfers a methyl group from S-adenosylmethionine to nicotinamide, forming N1-methylnicotinamide, a main route for vitamin B3 clearance. By consuming S-adenosylmethionine it can lower cellular methylation capacity and influence epigenetic marks. It is highly expressed in liver and adipose tissue and is studied in metabolism and cancer.
| Target | Nicotinamide N-methyltransferase (gene NNMT; UniProt P40261, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NNMT fragment (Lys8–Leu264), expressed in E. coli. Sequence identity with the mouse ortholog: 85.1%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nicotinamide N-methyltransferase in the cytoplasm. Tissue expression noted by UniProt (human): Predominantly expressed in the liver.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
